'sgRNA Online' is an online tool that identifies efficient sgRNAs for CRISPR editing of mouse and human genomes. It supports both classic Cas9 and xCa9 3.7.

20bp   18bp  


  • If you need to find optimized sgRNA in multiple sequences, please paste sequences in fasta format; Example
  • If your sequence(s) include repeated region/sequence, please mask them with "N"s, which will largely reduce the time cost;
  • Sequence requirements: a) Max sequence number: 10; b) Min sequence length >=23; c) Max sequence length <= 300;


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