'sgRNA Online' is an online tool that identifies efficient sgRNAs for CRISPR editing of mouse and human genomes. It supports both classic Cas9 and xCa9 3.7.

Information
Parameters
20bp   18bp  

Tips:

  • If you need to find optimized sgRNA in multiple sequences, please paste sequences in fasta format; Example
  • If your sequence(s) include repeated region/sequence, please mask them with "N"s, which will largely reduce the time cost;
  • Sequence requirements: a) Max sequence number: 10; b) Min sequence length >=23; c) Max sequence length <= 300;

 







2018 This web server is maintained by Casellas Lab at NIH/NIAMS
Developed & Designed by Yongbing Zhao